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Journal of Biomedical Optics • Open Access

Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media
Author(s): Mihaela Balu; Tommaso Baldacchini; John L. Carter; Tatiana B. Krasieva; Ruben Zadoyan; Bruce Jason Tromberg

Paper Abstract

We present a comparative study of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging in turbid media at 800- and 1300-nm excitation. The depth-dependent decay of TPEF and SHG signals in turbid tissue phantoms is used to estimate the impact of light scattering on excitation intensity at each wavelength. A 50 to 80% increase in scattering length is observed using 1300-nm excitation, while peak TPEF emission intensity is obtained 10 to 20 μm beneath the surface for both sources. The increased penetration depth at 1300 nm is confirmed by TPEF and SHG microscopy of tissue phantoms composed of gelatin/microspheres and 3-D organotypic collagen-fibroblast cultures, respectively. Our results establish the feasibility of 1.3-μm excitation in nonlinear optical microscopy.

Paper Details

Date Published: 1 January 2009
PDF: 3 pages
J. Biomed. Opt. 14(1) 010508 doi: 10.1117/1.3081544
Published in: Journal of Biomedical Optics Volume 14, Issue 1
Show Author Affiliations
Mihaela Balu, Univ. of California, Irvine (United States)
Tommaso Baldacchini, Newport Corp. (United States)
John L. Carter, Newport Corp. (United States)
Tatiana B. Krasieva, Univ. of California, Irvine (United States)
Ruben Zadoyan, Newport Corp. (United States)
Bruce Jason Tromberg, Univ. of California, Irvine (United States)


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