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Journal of Biomedical Optics

Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope
Author(s): Yu Ohsugi; Masataka Kinjo
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Paper Abstract

We report simultaneous determination of diffusion coefficients at different points of a cell membrane using a multipoint fluorescence correlation spectroscopy (FCS) system. A system carrying seven detection areas in the evanescent field is achieved by using seven optical fibers on the image plane in the detection port of an objective-type total internal reflection FCS (TIR-FCS) system. Fluctuation of fluorescence intensity is monitored and evaluated using seven photomultiplier tubes (PMTs) and a newly constructed multichannel correlator. We demonstrate simultaneous-multipoint FCS, with a 3-μs time resolution, to investigate heterogeneous structures such as cell membranes and membrane-binding molecular dynamics near glass surfaces in live cells.

Paper Details

Date Published: 1 January 2009
PDF: 4 pages
J. Biomed. Opt. 14(1) 014030 doi: 10.1117/1.3080723
Published in: Journal of Biomedical Optics Volume 14, Issue 1
Show Author Affiliations
Yu Ohsugi, Hokkaido Univ. (Japan)
Masataka Kinjo, Hokkaido Univ. (Japan)

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