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Journal of Biomedical Optics

Automated three-dimensional tracking of living cells by digital holographic microscopy
Author(s): Patrik Langehanenberg; Lyubomira Ivanova; Ingolf Bernhardt; Steffi Ketelhut; Angelika Vollmer; Dieter Dirksen; Georgi K. Georgiev; Gert von Bally; Björn Kemper
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Paper Abstract

Digital holographic microscopy (DHM) enables a quantitative multifocus phase contrast imaging that has been found suitable for technical inspection and quantitative live cell imaging. The combination of DHM with fast and robust autofocus algorithms enables subsequent automated focus realignment by numerical propagation of the digital holographically reconstructed object wave. In combination with a calibrated optical imaging system, the obtained propagation data quantify axial displacements of the investigated sample. The evaluation of quantitative DHM phase contrast images also enables an effective determination of lateral cell displacements. Thus, 3-D displacement data are provided. Results from investigations on sedimenting red blood cells and HT-1080 fibrosarcoma cells in a collagen tissue model demonstrate that DHM enables marker-free automated quantitative dynamic 3-D cell tracking without mechanical focus adjustment.

Paper Details

Date Published: 1 January 2009
PDF: 7 pages
J. Biomed. Opt. 14(1) 014018 doi: 10.1117/1.3080133
Published in: Journal of Biomedical Optics Volume 14, Issue 1
Show Author Affiliations
Patrik Langehanenberg, Universitätsklinikum Münster (Germany)
Lyubomira Ivanova, Univ. des Saarlandes (Germany)
Ingolf Bernhardt, Univ. des Saarlandes (Germany)
Steffi Ketelhut, Ctr. for Biomedical Optics and Photonics (Germany)
Angelika Vollmer, Ctr. for Biomedical Optics and Photonics (Germany)
Dieter Dirksen, Westfälische Wilhelms-Univ Münster (Germany)
Georgi K. Georgiev, Sofia Univ. "St. Kliment Ohridski" (Bulgaria)
Gert von Bally, Ctr. for Biomedical Optics and Photonics (Germany)
Björn Kemper, Ctr. for Biomedical Optics and Photonics (Germany)

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