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Journal of Biomedical Optics

Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniques
Author(s): Jun Liu; Constance Lay Lay Saw; Malini Olivo; Thankiah Sudhaharan; Sohail Ahmed; Paul Wan Sia Heng; Thorsten Wohland
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Paper Abstract

We report the detection of interactions between a photosensitizer, hypericin (HY), and its solvent system prepared with a formulation additive, polyvinylpyrrolidone (PVP), a commonly used pharmaceutical excipient. Fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging microscopy (FLIM) were used to study aggregation and binding of HY in the presence of PVP. Digitized fluorescence endoscopic imaging (DFEI) was used to study the effect of the pharmaceutical formulation in the in vivo tumor implanted chick chorioallantoic membrane (CAM) model. The results presented reveal the coordination of HY-PVP binding, HY disaggregation in the presence of PVP, and strengthened HY tumor uptake selectivity. PVP is thus suggested as a potential adjuvant to previously investigated N-methyl pyrrolidone (NMP) in the HY delivery system as well as a replacement for the conventionally used albumin in the HY bladder instillation fluids preparation for clinical use.

Paper Details

Date Published: 1 January 2009
PDF: 9 pages
J. Biomed. Opt. 14(1) 014003 doi: 10.1117/1.3067726
Published in: Journal of Biomedical Optics Volume 14, Issue 1
Show Author Affiliations
Jun Liu, NUS Graduate Programme in Bioengineering (Singapore)
Constance Lay Lay Saw, Rutgers Univ. (United States)
Malini Olivo, Singapore Bioimaging Consortium (Singapore)
Thankiah Sudhaharan
Sohail Ahmed, A*STAR Institute of Medical Biology (Singapore)
Paul Wan Sia Heng, National Univ. of Singapore (Singapore)
Thorsten Wohland, National Univ. of Singapore (Singapore)

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