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Journal of Biomedical Optics

Diagnosis of virus infection in orchid plants with high-resolution optical coherence tomography
Author(s): Tzu Hao Chow; Khay-Ming Eddie Tan; Beng-Koon Ng; Razul Sirajudeen Gulam; Chia Meng Tay; Tet Fatt Chia; Wee Tang Poh
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Paper Abstract

This work investigates the use of optical coherence tomography (OCT) to identify virus infection in orchid plants. Besides revealing the cross-sectional structure of orchid leaves, highly scattering upper leaf epidermides are detected with OCT for virus-infected plants. This distinct feature is not observable under histological examination of the leaf samples. Furthermore, the leaf epidermides of stressed but healthy plants, which exhibit similar visual symptoms as virus-infected plants, are not highly scattering and are similar to those of healthy plants. The results suggest that virus-infected orchid plants can be accurately identified by imaging the epidermal layers of their leaves with OCT. The OCT modality is suitable for fast, nondestructive diagnosis of orchid virus infection, which may potentially lead to significant cost savings and better control of the spread of viruses in the orchid industry.

Paper Details

Date Published: 1 January 2009
PDF: 6 pages
J. Biomed. Opt. 14(1) 014006 doi: 10.1117/1.3066900
Published in: Journal of Biomedical Optics Volume 14, Issue 1
Show Author Affiliations
Tzu Hao Chow, Nanyang Technological Univ. (Singapore)
Khay-Ming Eddie Tan, Univ. of St. Andrews (United Kingdom)
Beng-Koon Ng, Nanyang Technological Univ. (Singapore)
Razul Sirajudeen Gulam, Nanyang Technological Univ. (Singapore)
Chia Meng Tay, Nanyang Technological Univ. (Singapore)
Tet Fatt Chia, National Institute of Education (Singapore)
Wee Tang Poh, Changi General Hospital Pte Ltd. (Singapore)

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