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Journal of Biomedical Optics

Fluorescence spectroscopy of oral tissue: Monte Carlo modeling with site-specific tissue properties
Author(s): Ina Pavlova; Crystal Weber; Richard A. Schwarz; Michelle Diane Williams; Ann Marie Gillenwater; Rebecca R. Richards-Kortum
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Paper Abstract

A Monte Carlo model with site-specific input is used to predict depth-resolved fluorescence spectra from individual normal, inflammatory, and neoplastic oral sites. Our goal in developing this model is to provide a computational tool to study how the morphological characteristics of the tissue affect clinically measured spectra. Tissue samples from the measured sites are imaged using fluorescence confocal microscopy; autofluorescence patterns are measured as a function of depth and tissue sublayer for each individual site. These fluorescence distributions are used as input to the Monte Carlo model to generate predictions of fluorescence spectra, which are compared to clinically measured spectra on a site-by-site basis. A lower fluorescence intensity and longer peak emission wavelength observed in clinical spectra from dysplastic and cancerous sites are found to be associated with a decrease in measured fluorescence originating from the stroma or deeper fibrous regions, and an increase in the measured fraction of photons originating from the epithelium or superficial tissue layers. The simulation approach described here can be used to suggest an optical probe design that samples fluorescence at a depth that gives optimal separation in the spectral signal measured for benign, dysplastic, and cancerous oral mucosa.

Paper Details

Date Published: 1 January 2009
PDF: 10 pages
J. Biomed. Opt. 14(1) 014009 doi: 10.1117/1.3065544
Published in: Journal of Biomedical Optics Volume 14, Issue 1
Show Author Affiliations
Ina Pavlova, Cornell Univ. (United States)
Crystal Weber, Rice Univ. (United States)
Richard A. Schwarz, Rice Univ. (United States)
Michelle Diane Williams, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Ann Marie Gillenwater, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)

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