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Journal of Biomedical Optics

Two-photon imaging using adaptive phase compensated ultrashort laser pulses
Author(s): Peng Xi; Yair Andegeko; Dmitry Pestov; Vadim V. Lozovoy; Marcos M. Dantus
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Paper Abstract

An adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning (MIIPS) was used, together with a prism-pair, to measure and cancel high-order phase distortions introduced by a high-numerical-aperture objective and other dispersive elements of a two-photon laser-scanning microscope. The delivery of broad-bandwidth (~100 nm), sub-12-fs pulses was confirmed by interferometric autocorrelation measurements at the focal plane. A comparison of two-photon imaging with transform-limited and second-order-dispersion compensated laser pulses of the same energy showed a 6-to-11-fold improvement in the two-photon excitation fluorescence signal when applied to cells and tissue, and up to a 19-fold improvement in the second harmonic generation signal from a rat tendon specimen.

Paper Details

Date Published: 1 January 2009
PDF: 7 pages
J. Biomed. Opt. 14(1) 014002 doi: 10.1117/1.3059629
Published in: Journal of Biomedical Optics Volume 14, Issue 1
Show Author Affiliations
Peng Xi, Shanghai Jiao Tong Univ. (China)
Yair Andegeko, Michigan State Univ. (United States)
Dmitry Pestov, Michigan State Univ. (United States)
Vadim V. Lozovoy, Michigan State Univ. (United States)
Marcos M. Dantus, Michigan State Univ. (United States)


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