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Journal of Biomedical Optics

Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy
Author(s): Terry Alden Gaige; Hyuk-Sang Kwon; Guangping Dai; Victor C. Cabral; Ruopeng Wang; Yoon Sung Nam; Bevin P. Engelward; Van J. Wedeen; Peter T. C. So; Richard J. Gilbert
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Paper Abstract

The tongue consists of a complex, multiscale array of myofibers that comprise the anatomical underpinning of lingual mechanical function. 3-D myoarchitecture was imaged in mouse tongues with diffusion spectrum magnetic resonance imaging (DSI) at 9.4 T (bmax 7000 s/mm, 150-μm isotropic voxels), a method that derives the preferential diffusion of water/voxel, and high-throughput (10 fps) two-photon microscope (TPM). Net fiber alignment was represented for each method in terms of the local maxima of an orientational distribution function (ODF) derived from the local diffusion (DSI) and 3-D structural autocorrelation (TPM), respectively. Mesoscale myofiber tracts were generated by alignment of the principal orientation vectors of the ODFs. These data revealed a consistent relationship between the properties of the respective ODFs and the virtual superimposition of the distributed mesoscale myofiber tracts. The identification of a mesoscale anatomical construct, which specifically links the microscopic and macroscopic spatial scales, provides a method for relating the orientation and distribution of cells and subcellular components with overall tissue morphology, thus contributing to the development of multiscale methods for mechanical analysis.

Paper Details

Date Published: 1 November 2008
PDF: 8 pages
J. Biomed. Opt. 13(6) 064005 doi: 10.1117/1.3046724
Published in: Journal of Biomedical Optics Volume 13, Issue 6
Show Author Affiliations
Terry Alden Gaige, Athinoula A. Martinos Ctr for Biomedical Imaging (United States)
Hyuk-Sang Kwon, Massachusetts Institute of Technology (United States)
Guangping Dai, Athinoula A. Martinos Ctr for Biomedical Imaging (United States)
Victor C. Cabral, Massachusetts Institute of Technology Chapter (United States)
Ruopeng Wang, Athinoula A. Martinos Ctr for Biomedical Imaging (United States)
Yoon Sung Nam, Massachusetts Institute of Technology (United States)
Bevin P. Engelward, Massachusetts Institute of Technology (United States)
Van J. Wedeen, Athinoula A. Martinos Ctr for Biomedical Imaging (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Richard J. Gilbert, Massachusetts Institute of Technology (United States)


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