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Journal of Biomedical Optics • Open Access

Live imaging of blood flow in mammalian embryos using Doppler swept-source optical coherence tomography

Paper Abstract

Studying hemodynamic changes during early mammalian embryonic development is critical for further advances in prevention, diagnostics, and treatment of congenital cardiovascular (CV) birth defects and diseases. Doppler optical coherence tomography (OCT) has been shown to provide sensitive measurements of blood flow in avian and amphibian embryos. We combined Doppler swept-source optical coherence tomography (DSS-OCT) and live mouse embryo culture to analyze blood flow dynamics in early embryos. SS-OCT structural imaging was used for the reconstruction of embryo morphology and the orientation of blood vessels, which is required for calculating flow velocity from the Doppler measurements. Spatially and temporally resolved blood flow profiles are presented for the dorsal aorta and a yolk sac vessel in a 9.5-day embryo. We demonstrate that DSS-OCT can be successfully used for structural analysis and spatially and temporally resolved hemodynamic measurements in developing early mammalian embryos.

Paper Details

Date Published: 1 November 2008
PDF: 3 pages
J. Biomed. Opt. 13(6) 060506 doi: 10.1117/1.3046716
Published in: Journal of Biomedical Optics Volume 13, Issue 6
Show Author Affiliations
Irina V. Larina, The Univ. of Texas Medical Branch at Galveston (United States)
N. Sudheendran, Univ. of Houston (United States)
Mohamad G. Ghosn, Univ. of Houston (United States)
James Jiang, Thorlabs, Inc. (United States)
Alex Cable, Thorlabs, Inc. (United States)
Kirill V. Larin, Univ. of Houston (United States)
Mary Elizabeth Dickinson, Baylor College of Medicine (United States)


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