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Journal of Biomedical Optics

Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats
Author(s): Seonkyung Lee; Danthu H. Vu; Michael F. Hinds; Steven J. Davis; Alvin Liang; Tayyaba Hasan
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Paper Abstract

Photodynamic therapy (PDT) is a promising cancer treatment that involves optical excitation of photosensitizers that promote oxygen molecules to the metastable O2(a1Δ) state (singlet oxygen). This species is believed to be responsible for the destruction of cancerous cells during PDT. We describe a fiber optic-coupled, pulsed diode laser-based diagnostic for singlet oxygen. We use both temporal and spectral filtering to enhance the detection of the weak O2(a→X) emission near 1.27 µm. We present data that demonstrate real-time singlet oxygen production in tumor-laden rats with chlorin e6 and 5-aminolevulinic acid-induced protoporphyrin photosensitizers. We also observe a positive correlation between post-PDT treatment regression of the tumors and the relative amount of singlet oxygen measured. These results are promising for the development of the sensor as a real-time dosimeter for PDT.

Paper Details

Date Published: 1 November 2008
PDF: 8 pages
J. Biomed. Opt. 13(6) 064035 doi: 10.1117/1.3042265
Published in: Journal of Biomedical Optics Volume 13, Issue 6
Show Author Affiliations
Seonkyung Lee, Physical Sciences Inc. (United States)
Danthu H. Vu
Michael F. Hinds, Physical Sciences Inc. (United States)
Steven J. Davis, Physical Sciences Inc. (United States)
Alvin Liang, Wellman Ctr. for Photomedicine (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine (United States)

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