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Journal of Biomedical Optics • Open Access

Measuring erythrocyte deformability with fluorescence, fluid forces, and optical trapping
Author(s): Kapil Bambardekar; Aditya K. Dharmadhikari; Jayashree A. Dharmadhikari; Deepak Mathur; Shobhona Sharma

Paper Abstract

A laser-based method has been developed for experimentally probing single red blood cell (RBC) buckling and determining RBC membrane rigidity. Our method combines a liquid flow cell, fluorescence microscopy, and an optical-trap to facilitate simple measurements of the shear modulus and buckling properties of single RBCs, under physiological conditions. The efficacy of the method is illustrated by studying buckling behavior of normal and Plasmodium-infected RBCs, and the effect of Plasmodium falciparum–conditioned medium on normal, uninfected cells. Our simple method, which quantifies single-RBC deformability, may ease detection of RBC hematological disorders.

Paper Details

Date Published: 1 November 2008
PDF: 7 pages
J. Biomed. Opt. 13(6) 064021 doi: 10.1117/1.3037342
Published in: Journal of Biomedical Optics Volume 13, Issue 6
Show Author Affiliations
Kapil Bambardekar, Tata Institute of Fundamental Research (India)
Aditya K. Dharmadhikari, Tata Institute of Fundamental Research (India)
Jayashree A. Dharmadhikari, Tata Institute of Fundamental Research (India)
Deepak Mathur, Tata Institute of Fundamental Research (India)
Shobhona Sharma, Tata Institute of Fundamental Research (India)


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