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Journal of Biomedical Optics

Model-based analysis of reflectance and fluorescence spectra for in vivo detection of cervical dysplasia and cancer
Author(s): Crystal Elaine Redden Weber; Richard A. Schwarz; Edward Neely Atkinson; Dennis D. Cox; Calum E. MacAulay; Michele Follen; Rebecca R. Richards-Kortum
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Paper Abstract

Development, validation, and implementation of an analytical model to extract biologically and diagnostically relevant parameters from measured cervical tissue reflectance and fluorescence spectra are presented. Monte Carlo simulations of tissue reflectance are used to determine the relative contribution of the signal from the epithelium and stroma. The results indicate that the clinical probe used collects a majority of its reflectance signal from the stroma; therefore, a one-layer analytical model of reflectance is used. Two analytical approaches to calculate reflectance spectra are compared to Monte Carlo simulations, and a diffusion theory-based model is implemented. The model is validated by fitting spectra generated from Monte Carlo simulations and comparing the input and output parameters. Median agreement between extracted optical properties and input parameters is 10.6%. The reflectance model is used together with an analytical model of tissue fluorescence to extract optical properties and fluorophore concentrations from 748 clinical measurements of cervical tissue. A diagnostic algorithm based on these extracted parameters is developed and evaluated using cross-validation. The sensitivity/specificity of this algorithm relative to the gold standard of histopathology per measurement are 85/51%; this is comparable to accuracy reported in other studies of optical technologies for detection of cervical cancer and its precursors.

Paper Details

Date Published: 1 November 2008
PDF: 10 pages
J. Biomed. Opt. 13(6) 064016 doi: 10.1117/1.3013307
Published in: Journal of Biomedical Optics Volume 13, Issue 6
Show Author Affiliations
Crystal Elaine Redden Weber, Rice Univ. (United States)
Richard A. Schwarz, Rice Univ. (United States)
Edward Neely Atkinson, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Dennis D. Cox, Rice Univ. (United States)
Calum E. MacAulay, The BC Cancer Research Ctr. (Canada)
Michele Follen, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)


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