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Journal of Biomedical Optics

Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue: applications for diagnosis of oral precancer
Author(s): Ina Pavlova; Crystal Elaine Redden Weber; Richard A. Schwarz; Michelle Diane Williams; Adel K. El-Naggar; Ann Marie Gillenwater; Rebecca R. Richards-Kortum
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Paper Abstract

We present a Monte Carlo model to predict fluorescence spectra of the oral mucosa obtained with a depth-selective fiber optic probe as a function of tissue optical properties. A model sensitivity analysis determines how variations in optical parameters associated with neoplastic development influence the intensity and shape of spectra, and elucidates the biological basis for differences in spectra from normal and premalignant oral sites. Predictions indicate that spectra of oral mucosa collected with a depth-selective probe are affected by variations in epithelial optical properties, and to a lesser extent, by changes in superficial stromal parameters, but not by changes in the optical properties of deeper stroma. The depth selective probe offers enhanced detection of epithelial fluorescence, with 90% of the detected signal originating from the epithelium and superficial stroma. Predicted depth-selective spectra are in good agreement with measured average spectra from normal and dysplastic oral sites. Changes in parameters associated with dysplastic progression lead to a decreased fluorescence intensity and a shift of the spectra to longer emission wavelengths. Decreased fluorescence is due to a drop in detected stromal photons, whereas the shift of spectral shape is attributed to an increased fraction of detected photons arising in the epithelium.

Paper Details

Date Published: 1 November 2008
PDF: 13 pages
J. Biomed. Opt. 13(6) 064012 doi: 10.1117/1.3006066
Published in: Journal of Biomedical Optics Volume 13, Issue 6
Show Author Affiliations
Ina Pavlova, Cornell Univ. (United States)
Crystal Elaine Redden Weber, Rice Univ. (United States)
Richard A. Schwarz, Rice Univ. (United States)
Michelle Diane Williams, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Adel K. El-Naggar, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Ann Marie Gillenwater, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)

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