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Journal of Biomedical Optics

High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas
Author(s): Tomas Alanentalo; Christina E. Loren; Asa Larefalk; James Sharpe; Dan Holmberg; Ulf Ahlgren
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Paper Abstract

A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required.

Paper Details

Date Published: 1 September 2008
PDF: 4 pages
J. Biomed. Opt. 13(5) 054070 doi: 10.1117/1.3000430
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Tomas Alanentalo, Umeå Univ. (Sweden)
Christina E. Loren, Umeå Univ. (Sweden)
Asa Larefalk, Umeå Univ. (Sweden)
James Sharpe, Ctr. for Genomic Regulation (Spain)
Dan Holmberg, Umeå Univ. (Sweden)
Ulf Ahlgren, Umeå Univ. (Sweden)


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