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Journal of Biomedical Optics

Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers
Author(s): T. J. Harvey; Elsa Correia Faria; Alex Henderson; Ehsan Gazi; A. D. Ward; N. W. Clarke; Mick D. Brown; Richard D. Snook; Peter Gardner
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Paper Abstract

An investigation into the use of Raman optical tweezers to study urological cell lines is reported, with the ultimate aim of determining the presence of malignant CaP cells in urine and peripheral fluids. To this end, we trapped and analyzed live CaP cells (PC-3) and bladder cells (MGH-U1), because both prostate and bladder cells are likely to be present in urine. The laser excitation wavelength of 514.5 nm was used, with Raman light collected both in back- and forward-scattering geometric configurations. For the backscattering configuration the same laser was used for trapping and excitation, while for forward scattering a 1064 nm laser provided the trapping beam. Analysis of cell-diameter distributions for cells analyzed suggested normal distribution of cell sizes, indicating an unbiased cell-selection criterion. Principal components analysis afforded discrimination of MGH-U1 and PC-3 spectra collected in either configuration, demonstrating that it is possible to trap, analyze, and differentiate PC-3 from MGH-U1 cells using a 514.5 nm laser. By loading plot analysis, possible biomolecules responsible for discrimination in both configurations were determined. Finally, the effect of cell size on discrimination was investigated, with results indicating that separation is based predominantly on cell type rather than cell size.

Paper Details

Date Published: 1 November 2008
PDF: 12 pages
J. Biomed. Opt. 13(6) 064004 doi: 10.1117/1.2999609
Published in: Journal of Biomedical Optics Volume 13, Issue 6
Show Author Affiliations
T. J. Harvey, Manchester Interdisciplinary Biocentre (United Kingdom)
Elsa Correia Faria, Manchester Interdisciplinary Biocentre (United Kingdom)
Alex Henderson, Manchester Interdisciplinary Biocentre (United Kingdom)
Ehsan Gazi, The Paterson Institute for Cancer Research (United Kingdom)
A. D. Ward, Science and Technology Facilities Council (United Kingdom)
N. W. Clarke, The Paterson Institute for Cancer Research (United Kingdom)
Mick D. Brown, The Paterson Institute for Cancer Research (United Kingdom)
Richard D. Snook, Manchester Interdisciplinary Biocentre (United Kingdom)
Peter Gardner, Manchester Interdisciplinary Biocentre (United Kingdom)

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