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Journal of Biomedical Optics • Open Access

Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery

Paper Abstract

The ability to inject exogenous material as well as to alter subcellular structures in a minimally invasive manner using a laser microbeam has been useful for cell biologists to study the structure-function relationship in complex biological systems. We describe a quantitative phase laser microsurgery system, which takes advantage of the combination of laser microirradiation and short-coherence interference microscopy. Using this method, quantitative phase images and the dynamic changes of phase during the process of laser microsurgery of red blood cells (RBCs) can be evaluated in real time. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intracellular structures, being exposed to the laser microbeam. Such quantitation was not possible using conventional phase-contrast microscopy.

Paper Details

Date Published: 1 September 2008
PDF: 3 pages
J. Biomed. Opt. 13(5) 050508 doi: 10.1117/1.2997375
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Lingfeng Yu, Univ. of California, Irvine (United States)
Samarendra Kumar Mohanty, Raja Ramanna Ctr. for Advanced Technology (India)
Gangjun Liu, Beckman Laser Institute and Medical Clinic (United States)
Suzanne Genc, Beckman Laser Institute and Medical Clinic (United States)
Zhongping Chen, Univ. of California, Irvine (United States)
Michael W. Berns, Univ. of California, Irvine (United States)

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