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Journal of Biomedical Optics

Automated quantification of colonic crypt morphology using integrated microscopy and optical coherence tomography
Author(s): Xin Qi; Yinsheng Pan; Zhilin Hu; Wei Kang; Joseph E. Willis; Kayode Olowe; Michael V. Sivak; Andrew M. Rollins
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Paper Abstract

Colonic crypt morphological patterns have shown a close correlation with histopathological diagnosis. Imaging technologies such as high-magnification chromoendoscopy and endoscopic optical coherence tomography (OCT) are capable of visualizing crypt morphology in vivo. We have imaged colonic tissue in vitro to simulate high-magnification chromoendoscopy and endoscopic OCT and demonstrate quantification of morphological features of colonic crypts using automated image analysis. 2-D microscopic images with methylene blue staining and correlated 3-D OCT volumes were segmented using marker-based watershed segmentation. 2-D and 3-D crypt morphological features were quantified. The accuracy of segmentation was validated, and measured features are in agreement with known crypt morphology. This work can enable studies to determine the clinical utility of high-magnification chromoendoscopy and endoscopic OCT, as well as studies to evaluate crypt morphology as a biomarker for colonic disease progression.

Paper Details

Date Published: 1 September 2008
PDF: 11 pages
J. Biomed. Opt. 13(5) 054055 doi: 10.1117/1.2993323
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Xin Qi, Case Western Reserve Univ. (United States)
Yinsheng Pan, Case Western Reserve Univ. (United States)
Zhilin Hu, Case Western Reserve Univ. (United States)
Wei Kang, Case Western Reserve Univ. (United States)
Joseph E. Willis, Case Western Reserve Univ. (United States)
Kayode Olowe, Case Western Reserve Univ. (United States)
Michael V. Sivak, Case Western Reserve Univ. (United States)
Andrew M. Rollins, Case Western Reserve Univ. (United States)


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