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Journal of Biomedical Optics • Open Access

Beyond the diffraction-limit biological imaging by saturated excitation microscopy

Paper Abstract

We demonstrate high-resolution fluorescence imaging in biological samples by saturated excitation (SAX) microscopy. In this technique, we saturate the population of fluorescence molecules at the excited state with high excitation intensity to induce strong nonlinear fluorescence responses in the center of laser focus, which contributes the improvement of the spatial resolution in three dimensions. Using SAX microscopy, we observed stained microtubules in HeLa cells with improved spatial resolution. We also measured the relation of the fluorescence and excitation intensity with several kinds of fluorescence dyes and, in the results, confirmed that SAX microscopy has the potential to observe any kind of fluorescence samples in current usage.

Paper Details

Date Published: 1 September 2008
PDF: 3 pages
J. Biomed. Opt. 13(5) 050507 doi: 10.1117/1.2992595
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Masahito Yamanaka, Osaka Univ. (Japan)
Shogo Kawano, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Nicholas Isaac Smith, Osaka Univ. (Japan)
Satoshi Kawata, Osaka Univ. (Japan)

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