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Journal of Biomedical Optics

Optimization of fluorescence enhancement for silicon-based microarrays
Author(s): Vanessa Marino; Clelia Galati; Claudio Arnone
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Paper Abstract

An optical technique for the enhancement of fluorescence detection sensitivity on planar samples is presented. Such a technique is based on the simultaneous optimization of excitation and light collection by properly combining interference and reflectance from the sample holder. Comparative tests have been performed in microarray applications, by evaluating the proposed solution against commercial glass-based devices, using popular labeling dyes, such as Cy3 and Cy5. The proposed technique is implemented on a substrate built with standard silicon technology and is therefore well suited for integrated micro total analysis systems (µTAS) applications.

Paper Details

Date Published: 1 September 2008
PDF: 6 pages
J. Biomed. Opt. 13(5) 054060 doi: 10.1117/1.2992142
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Vanessa Marino, Univ. degli Studi di Palermo (Italy)
Clelia Galati, STMicroelectronics (Italy)
Claudio Arnone, Univ. degli Studi di Palermo (Italy)

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