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Journal of Biomedical Optics

Design and optimization of a high-speed, high-sensitivity, spinning disk confocal microscopy system
Author(s): Ryan McAllister; Daniel R. Sisan; Jeffrey S. Urbach
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Paper Abstract

We describe the principles, design, and systems integration of a flexible, high-speed, high-sensitivity, high-resolution confocal spinning disk microscopy (SDCM) system. We present several artifacts unique to high-speed SDCM along with techniques to minimize them. We show example experimental results from a specific implementation capable of generating 3-D image stacks containing 30 2-D slices at 30 stacks per second. This implementation also includes optics for differential interference contrast (DIC), phase, and bright-field imaging, as well as an optical trap with sensitive force and position measurement.

Paper Details

Date Published: 1 September 2008
PDF: 7 pages
J. Biomed. Opt. 13(5) 054058 doi: 10.1117/1.2992141
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Ryan McAllister, Georgetown Univ. (United States)
Daniel R. Sisan, Georgetown Univ. (United States)
Jeffrey S. Urbach, Georgetown Univ. (United States)


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