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Journal of Biomedical Optics

Light scattering by neutrophils: model, simulation, and experiment
Author(s): Darya Yu. Orlova; Maxim A. Yurkin; Alfons G. Hoekstra; Valerii Pavlovich Maltsev
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Paper Abstract

We studied the elastic light-scattering properties of human blood neutrophils, both experimentally and theoretically. The experimental study was performed with a scanning flow cytometer measuring the light-scattering patterns (LSPs) of individual cells over an angular range of 5–60 deg. We determined the absolute differential light-scattering cross sections of neutrophils. We also proposed an optical model for a neutrophil as a sphere filled by small spheres and prolate spheroids that correspond to granules and segmented nucleus, respectively. This model was used in simulations of LSPs using the discrete dipole approximation and different compositions of internal organelles. A comparison of experimentally measured and simulated LSPs gives a good qualitative agreement in LSP shape and quantitative agreement in overall magnitude of the differential light-scattering cross section.

Paper Details

Date Published: 1 September 2008
PDF: 7 pages
J. Biomed. Opt. 13(5) 054057 doi: 10.1117/1.2992140
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Darya Yu. Orlova, Institute of Chemical Kinetics and Combustion (Russian Federation)
Maxim A. Yurkin, Institute of Chemical Kinetics and Combustion (Russian Federation)
Alfons G. Hoekstra, Univ. van Amsterdam (Netherlands)
Valerii Pavlovich Maltsev, Institute of Chemical Kinetics and Combustion (Russian Federation)

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