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Journal of Biomedical Optics • Open Access

Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies
Author(s): Han-Wen Guo; Chien-Tsun Chen; Yau-Huei Wei; Oscar K. Lee; Vladimir Ghukasyan; Fu-Jen Kao; Hsing-Wen Wang

Paper Abstract

The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

Paper Details

Date Published: 1 September 2008
PDF: 3 pages
J. Biomed. Opt. 13(5) 050505 doi: 10.1117/1.2990752
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Han-Wen Guo, National Yang-Ming Univ. (Taiwan)
Chien-Tsun Chen, National Yang-Ming Univ. (Taiwan)
Yau-Huei Wei, National Yang-Ming Univ. (Taiwan)
Oscar K. Lee, National Yang-Ming Univ. (Taiwan)
Vladimir Ghukasyan, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)
Hsing-Wen Wang, National Yang-Ming Univ. (Taiwan)


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