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Journal of Biomedical Optics

Nonlinear optical microscopy of the bronchus
Author(s): Shuangmu Zhuo; Jianxin Chen; Biying Yu; Xingshan Jiang; Teng Leo; Quangang Liu; Rong Chen; Shusen Xie
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Paper Abstract

Because of frequent exposure to carcinogens, the bronchus is prone to early pathologic alterations. The assessment of these early changes is of key significance in physiological studies and disease diagnosis of the bronchus. We utilize nonlinear optical microscopy (NLOM) to image mouse bronchial tissue based on intrinsic nonlinear optical contrast. Our results show that NLOM is effective for imaging the bronchial intact microstructural components, providing quantitative information about the biomorphology and biochemistry of tissue. Our findings also display that NLOM can provide a two-photon ratiometric redox fluorometry, based on mitochondrial signals and reduced pyridine nucleotide (NADH and NADPH) and oxidized flavoproteins (Fp) signals, to assess the metabolic state of the epithelial cells and chondrocytes. It was found that NLOM can offer a sensitive tool, based on the second-harmonic signal depth-dependent decay, to obtain quantitative information on the optical property of the stroma associated with normal and diseased tissue states. Our results suggest that with the advent of the clinical portability of typical nonlinear optical endoscopy, the NLOM technique has the potential to be applied in vivo to the clinical diagnosis and monitoring of bronchial disease.

Paper Details

Date Published: 1 September 2008
PDF: 7 pages
J. Biomed. Opt. 13(5) 054024 doi: 10.1117/1.2982534
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Shuangmu Zhuo, Fujian Normal Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)
Biying Yu, Fujian Normal Univ. (China)
Xingshan Jiang, Fujian Normal Univ. (China)
Teng Leo, Fujian Normal Univ. (China)
Quangang Liu, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)


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