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Journal of Biomedical Optics

In vitro model that approximates retinal damage threshold trends
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Paper Abstract

Without effective in vitro damage models, advances in our understanding of the physics and biology of laser-tissue interaction would be hampered due to cost and ethical limitations placed on the use of nonhuman primates. We extend our characterization of laser-induced cell death in an existing in vitro retinal model to include damage thresholds at 514 and 413 nm. The new data, when combined with data previously reported for 532 and 458 nm exposures, provide a sufficiently broad range of wavelengths and exposure durations (0.1 to 100 s) to make comparisons with minimum visible lesion (in vivo) data in the literature. Based on similarities between in vivo and in vitro action spectra and temporal action profiles, the cell culture model is found to respond to laser irradiation in a fundamentally similar fashion as the retina of the rhesus animal model. We further show that this response depends on the amount of intracellular melanin pigmentation.

Paper Details

Date Published: 1 September 2008
PDF: 6 pages
J. Biomed. Opt. 13(5) 054014 doi: 10.1117/1.2981831
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Michael L. Denton, Northrop Grumman Mission Systems (United States)
Michael S. Foltz, Northrop Grumman Mission Systems (United States)
Kurt J. Schuster, Northrop Grumman Mission Systems (United States)
Gary D. Noojin, Northrop Grumman Mission Systems (United States)
Larry E. Estlack, Conceptual MindWorks, Inc. (United States)
Robert J. Thomas, Air Force Research Lab. (United States)

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