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Journal of Biomedical Optics

Endoscopic image analysis of photosensitizer fluorescence as a promising noninvasive approach for pathological grading of bladder cancer in situ
Author(s): James Chen-Yong Kah; Weber K. O. Lau; Phuay Hoon Tan; Colin James Richard Sheppard; Malini Olivo
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Paper Abstract

Our aim is to apply image analysis on photosensitizer fluorescence and compare the relationship between histopathology and endoscopic fluorescence imaging. The correlation between hypericin fluorescence and histopathology of diseased tissue was explored in a clinical study involving 58 fluorescence cystoscopic images from 23 patients. Based on quantification of fluorescence colorimetric parameters extracted from the image analysis, diagnostic functions were developed to pathologically classify the bladder cancer. Our preliminary results show that the differences in fluorescence intensity ratios among the three different grades of bladder cancer are statistically significant. The results also show a decrease in macroscopic fluorescence intensity that correlated with higher cancer grades. By combining both the red-to-green and red-to-blue fluorescence intensity ratios into a 2-D scatter plot and defining diagnostic linear discrimination functions on the data points, this technique is able to yield an average sensitivity and specificity of around 68.6% and 86.1%, respectively, for pathological cancer grading of the three different grades of bladder cancer in our study. We conclude that our proposed approach in applying colorimetric intensity ratio analysis on hypericin fluorescence shows potential to optically grade bladder cancer in situ.

Paper Details

Date Published: 1 September 2008
PDF: 9 pages
J. Biomed. Opt. 13(5) 054022 doi: 10.1117/1.2981827
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
James Chen-Yong Kah, National Univ. of Singapore (Singapore)
Weber K. O. Lau, Singapore General Hospital (Singapore)
Phuay Hoon Tan, Singapore General Hospital (Singapore)
Colin James Richard Sheppard, National Univ. of Singapore (Singapore)
Malini Olivo, National Cancer Ctr. of Singapore (Singapore)

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