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Journal of Biomedical Optics • Open Access

Label-free imaging of Drosophila larva by multiphoton autofluorescence and second harmonic generation microscopy

Paper Abstract

The fruit fly Drosophila melanogaster is one of the most valuable organisms in studying genetics and developmental biology. To gain insight into Drosophila development, we successfully acquired label-free, in vivo images of both developing muscles and internal organs in a stage 2 larva using the minimally invasive imaging modality of multiphoton autofuorescence (MAF) and second harmonic generation (SHG) microscopy. We found that although MAF is useful in identifying structures such as the digestive system, trachea, and intestinal track, it is the SHG signal that allowed the investigation of the muscular architecture within the developing larva. Our results suggest that multiphoton microscopy is a powerful in vivo, label-free imaging technique to examine Drosophila physiology and may be used for developmental studies.

Paper Details

Date Published: 1 September 2008
PDF: 3 pages
J. Biomed. Opt. 13(5) 050502 doi: 10.1117/1.2981817
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Chiao-Ying Lin, National Taiwan Univ. (Taiwan)
Vladimir A. Hovhannisyan, National Taiwan Univ. (Taiwan)
June-Tai Wu, National Taiwan Univ. Hospital (Taiwan)
Chii-Wann Lin, National Taiwan Univ. (Taiwan)
Jyh-Horng Chen, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


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