Share Email Print

Journal of Biomedical Optics

Noninvasive photoacoustic identification of sentinel lymph nodes containing methylene blue in vivo in a rat model
Author(s): Kwang Hyun Song; Erich W. Stein; Julie A. Margenthaler; Lihong V. Wang
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

Sentinel lymph node biopsy (SLNB) has become the standard method of axillary staging for patients with breast cancer and clinically negative axillae. Even though SLNB using both methylene blue and radioactive tracers has a high identification rate, it still relies on an invasive surgical procedure with associated morbidity. Axillary ultrasound has emerged as a diagnostic tool to evaluate the axilla, but it can only assess morphology and cannot specifically identify sentinel lymph nodes (SLNs). In this pilot study, we propose a noninvasive photoacoustic SLN identification system using methylene blue injection in a rat model. We successfully image a SLN with high optical contrast (146±41, standard deviation) and good ultrasonic resolution (~500 μm) in vivo. We also show potential feasibility for clinical applications by imaging 20- and 31-mm-deep SLNs in 3-D and 2-D, respectively. Our results suggest that this technology would be a useful clinical tool, allowing clinicians to identify SLNs noninvasively in vivo.

Paper Details

Date Published: 1 September 2008
PDF: 6 pages
J. Biomed. Opt. 13(5) 054033 doi: 10.1117/1.2976427
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Kwang Hyun Song, Texas A&M Univ. (United States)
Erich W. Stein, Washington Univ. in St. Louis (United States)
Julie A. Margenthaler, Washington Univ. in St. Louis School of Medicine (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

© SPIE. Terms of Use
Back to Top