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Journal of Biomedical Optics • Open Access

Role of cervicitis in the Raman-based optical diagnosis of cervical intraepithelial neoplasia
Author(s): Herculano da Silva Martinho; Claudia Maria de Oliveira Monteiro da Silva; Maria Christina Botelho Mendonca Yassoyama; Patricia de Oliveira Andrade; Renata Andrade Bitar; Ana Maria Do Espirito Santo; Emilia Angela Loschiavo Arisawa; Airton Abrahao Martin

Paper Abstract

The Raman-based optical diagnosis of normal cervix, inflammative cervix (cervicitis), and cervical intraepithelial neoplasia was investigated on samples of 63 patients. The main alterations were found in the 857 cm-1 (CCH deformation aromatic); 925 cm-1 (C–C stretching); ~1247 cm-1 (CN stretch, NH bending of Amide III); 1370 cm-1 (CH2 bending); and 1525 cm-1 (C=C/C=N stretching) vibrational bands in accordance with previously reported in the literature comparing normal and malignant cervical tissue. The statistical analysis (principal components analysis, clustering, and logistic regression models) applied to the spectral data indicated that the full discrimination among normal and neoplastic tissues of cervix by Raman optical biopsy is seriously affected by the presence of inflammatory infiltrates, which increases the false-positive rate. This fact is specially relevant once cervicitis is a very common state (noncancerous) of the cervix of sexually active woman. The results suggest that, for the correct Raman-based diagnosis of normal cervix from cervical intraepithelial neoplasia, it is necessary to use an auxiliary way to discriminate the contribution from the inflammatory infiltrates.

Paper Details

Date Published: 1 September 2008
PDF: 6 pages
J. Biomed. Opt. 13(5) 054029 doi: 10.1117/1.2976114
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
Herculano da Silva Martinho, Univ. do Vale do Paraíba (Brazil)
Claudia Maria de Oliveira Monteiro da Silva, Univ. do Vale do Paraíba (Brazil)
Maria Christina Botelho Mendonca Yassoyama, Univ. do Vale do Paraíba (Brazil)
Patricia de Oliveira Andrade, Univ. do Vale do Paraíba (Brazil)
Renata Andrade Bitar, Univ. do Vale do Paraíba (Brazil)
Ana Maria Do Espirito Santo, Univ. do Vale do Paraíba (Brazil)
Emilia Angela Loschiavo Arisawa, Univ. do Vale do Paraíba (Brazil)
Airton Abrahao Martin, Univ. do Vale do Paraíba (Brazil)

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