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Journal of Biomedical Optics • Open Access

Bimodal biophotonic imaging of the structure-function relationship in cardiac tissue
Author(s): William J. Hucker; Crystal M. Ripplinger; Christine P. Fleming; Vadim V. Fedorov; Andrew M. Rollins; Igor R. Efimov

Paper Abstract

The development of systems physiology is hampered by the limited ability to relate tissue structure and function in intact organs in vivo or in vitro. Here, we show the application of a bimodal biophotonic imaging approach that employs optical coherence tomography and fluorescent imaging to investigate the structure-function relationship at the tissue level in the heart. Reconstruction of cardiac excitation and structure was limited by the depth penetration of bimodal imaging to ~2 mm in atrial tissue, and ~1 mm in ventricular myocardium. The subcellular resolution of optical coherence tomography clearly demonstrated that microscopic fiber orientation governs the pattern of wave propagation in functionally characterized rabbit sinoatrial and atrioventricular nodal preparations and revealed structural heterogeneities contributing to ventricular arrhythmias. The combination of this bimodal biophotonic imaging approach with histology and/or immunohistochemistry can span multiple scales of resolution for the investigation of the molecular and structural determinants of intact tissue physiology.

Paper Details

Date Published: 1 September 2008
PDF: 7 pages
J. Biomed. Opt. 13(5) 054012 doi: 10.1117/1.2975826
Published in: Journal of Biomedical Optics Volume 13, Issue 5
Show Author Affiliations
William J. Hucker, Washington Univ. in St. Louis (United States)
Crystal M. Ripplinger, Washington Univ. in St. Louis (United States)
Christine P. Fleming, Case Western Reserve Univ. (United States)
Vadim V. Fedorov, Washington Univ. in St. Louis (United States)
Andrew M. Rollins, Case Western Reserve Univ. (United States)
Igor R. Efimov, Washington Univ. in St. Louis (United States)

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