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Journal of Biomedical Optics

Fluorescence tomography technique optimized for noninvasive imaging of the mouse brain
Author(s): Riad Bourayou; Heide Boeth; Heval Benav; Thomas Betz; Ute Lindauer; Till Nierhaus; Jan Klohs; Andreas Wunder; Ulrich Dirnagl; Jens M. Steinbrink
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Paper Abstract

In vivo molecular fluorescence tomography of brain disease mouse models has two very specific demands on the optical setup: the use of pigmented furry mice does not allow for a purely noncontact setup, and a high spatial accuracy is required on the dorsal side of the animal due to the location of the brain. We present an optimized setup and tomographic scheme that meet these criteria through a combined CW reflectance-transmittance fiber illumination approach and a charge-coupled device contactless detection scheme. To consider the anatomy of the mouse head and take short source detector separations into account, the forward problem was evaluated by a Monte Carlo simulation input with a magnetic resonance image of the animal. We present an evaluation of reconstruction performance of the setup under three different condition. (i) Using a simulated dataset, with well-defined optical properties and low noise, the reconstructed position accuracy is below 0.5 mm. (ii) Using experimental data on a cylindrical tissue-simulating phantom with well-defined optical properties, a spatial accuracy of about 1 mm was found. (iii) Finally, on an animal model with a fluorescent inclusion in the brain, the target position was reconstructed with an accuracy of 1.6 mm.

Paper Details

Date Published: 1 July 2008
PDF: 8 pages
J. Biomed. Opt. 13(4) 041311 doi: 10.1117/1.2968262
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Riad Bourayou, Charité Universitätsmedizin Berlin (Germany)
Heide Boeth, Charité Universitätsmedizin Berlin (Germany)
Heval Benav, Charité Universitätsmedizin Berlin (Germany)
Thomas Betz, Charité Universitätsmedizin Berlin (Germany)
Ute Lindauer, Charité Universitätsmedizin Berlin (Germany)
Till Nierhaus, Charité Universitätsmedizin Berlin (Germany)
Jan Klohs, Charité Universitätsmedizin Berlin (Germany)
Andreas Wunder, Charité Universitätsmedizin Berlin (Germany)
Ulrich Dirnagl, Charité Universitätsmedizin Berlin (Germany)
Jens M. Steinbrink, Charité Universitätsmedizin Berlin (Germany)

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