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Journal of Biomedical Optics

Monitoring of hexyl 5-aminolevulinate-induced photodynamic therapy in rat bladder cancer by optical spectroscopy
Author(s): Eivind L. P. Larsen; Lise Lyngsnes Randeberg; Odrun Arna Gederaas; Carl-Jørgen Arum; Astrid Hjelde; Chun-Mei Zhao; Duan Chen; Hans E. Krokan; Lars Othar Svaasand
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Paper Abstract

Monitoring of the tissue response to photodynamic therapy (PDT) can provide important information to help optimize treatment variables such as drug and light dose, and possibly predict treatment outcome. A urinary bladder cancer cell line (AY-27) was used to induce orthotopic transitional cell carcinomas (TCC) in female Fischer rats, and hexyl 5-aminolevulinate (HAL, 8 mM, 1 h)-induced PDT was performed on day 14 after instillation of the cancer cells (20 J/cm2 fluence at 635 nm). In vivo optical reflectance and fluorescence spectra were recorded from bladders before and after laser treatment with a fiberoptic probe. Calculated fluorescence bleaching and oxygen saturation in the bladder wall were examined and correlated to histology results. Reflectance spectra were analyzed using a three-layer optical photon transport model. Animals with TCC treated with PDT showed a clear treatment response; decreased tissue oxygenation and protoporphyrin IX (PpIX) fluorescence photobleaching were observed. Histology demonstrated that 3 of 6 animals with treatment had no sign of the tumor 7 days after PDT treatment. The other 3 animals had significantly reduced the tumor size. The most treatment-responsive animals had the highest PpIX fluorescence prior to light irradiation. Thus, optical spectroscopy can provide useful information for PDT. The model has proved to be very suitable for bladder cancer studies.

Paper Details

Date Published: 1 July 2008
PDF: 9 pages
J. Biomed. Opt. 13(4) 044031 doi: 10.1117/1.2967909
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Eivind L. P. Larsen, Norwegian Univ. of Science and Technology (Norway)
Lise Lyngsnes Randeberg, Norwegian Univ. of Science and Technology (Norway)
Odrun Arna Gederaas, Norwegian Univ. of Science and Technology (Norway)
Carl-Jørgen Arum, St. Olavs Hospital (Norway)
Astrid Hjelde, Norwegian Univ. of Science and Technology (Norway)
Chun-Mei Zhao, Norwegian Univ. of Science and Technology (Norway)
Duan Chen, Norwegian Univ. of Science and Technology (Norway)
Hans E. Krokan, Norwegian Univ. of Science and Technology (Norway)
Lars Othar Svaasand, Norwegian Univ. of Science and Technology (Norway)


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