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Journal of Biomedical Optics • Open Access

Intravital high-resolution optical imaging of individual vessel response to photodynamic treatment

Paper Abstract

Intravital imaging using confocal microscopy facilitates high-resolution studies of cellular and molecular events in vivo. We use this, complemented by Doppler optical coherence tomography (OCT), to assess blood flow in a mouse dorsal skin-fold window chamber model to image the response of individual blood vessels to localized photodynamic therapy (PDT). Specific fluorescent cell markers were used to assess the effect on the vascular endothelial cell lining of the treated vessels. A fluorescently tagged antibody against an endothelial transmembrane glycoprotein (CD31) was used to image endothelial cell integrity in the targeted blood vessel. A cell permeability (viability) indicator, SYTOX Orange, was also used to further assess damage to endothelial cells. A fluorescently labeled anti-CD41 antibody that binds to platelets was used to confirm platelet aggregation in the treated vessel. These optical techniques enable dynamic assessment of responses to PDT in vivo, at both the vascular endothelial cell and whole vessel levels.

Paper Details

Date Published: 1 July 2008
PDF: 3 pages
J. Biomed. Opt. 13(4) 040502 doi: 10.1117/1.2965545
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Mamta Khurana, Princess Margaret Hospital (Canada)
Eduardo Hiroyuki Moriyama, Princess Margaret Hospital (Canada)
Adrian Mariampillai, Princess Margaret Hospital (Canada)
Brian C. Wilson, Princess Margaret Hospital (Canada)

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