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Journal of Biomedical Optics

Multifunctional darkfield microscopy using an axicon
Author(s): Ming Lei; Baoli Yao
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Paper Abstract

We present a multifunctional darkfield microscopy using an axicon. It combines the functions of a darkfield microscope, fluorescence microscope, and microspectrophotometer in one platform. The advantage of the system over conventional darkfield microscopy includes the high transmittance of the illuminating flux, the high contrast of the image, and the convenient toggle between darkfield and brightfield microscopy. Examples of dark, bright, and fluorescent micrographs as well as concerned spectra of microsized specimens implemented in this apparatus are demonstrated.

Paper Details

Date Published: 1 July 2008
PDF: 4 pages
J. Biomed. Opt. 13(4) 044024 doi: 10.1117/1.2960019
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Ming Lei, Xi'an Institute of Optics and Precision Mechanics (China)
Baoli Yao, Xi'an Institute of Optics and Precision Mechanics (China)


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