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Journal of Biomedical Optics

Fluorescence diffuse tomography for detection of red fluorescent protein expressed tumors in small animals
Author(s): Ilya V. Turchin; Vladislav Antonievich Kamensky; Vladimir I. Plehanov; Anna Gennadjevna Orlova; Mikhail S. Kleshnin; Ilya I. Fiks; Marina V. Shirmanova; Irina G. Meerovich; Lyaisan R. Arslanbaeva; Viktoria V. Jerdeva; Alexander P. Savitsky
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Paper Abstract

A fluorescence diffuse tomography (FDT) setup for monitoring tumor growth in small animals has been created. In this setup an animal is scanned in the transilluminative configuration by a single source and detector pair. To remove stray light in the detection system, we used a combination of interferometric and absorption filters. To reduce the scanning time, an experimental animal was scanned using the following algorithm: (1) large-step scanning to obtain a general view of the animal (source and detector move synchronously); (2) selection of the fluorescing region; and (3) small-step scanning of the selected region and different relative shifts between the source and detector to obtain sufficient information for 3D reconstruction. We created a reconstruction algorithm based on the Holder norm to estimate the fluorophore distribution. This algorithm converges to the solution with a minimum number of fluorescing zones. The use of tumor cell lines transfected with fluorescent proteins allowed us to conduct intravital monitoring studies. Cell lines of human melanomas Mel-P, Mel-Ibr, Mel-Kor, and human embryonic kidney HEK293 Phoenix were transfected with DsRed-Express and Turbo-RFP genes. The emission of red fluorescent proteins (RFPs) in the long-wave optical range permits detection of deep-seated tumors. In vivo experiments were conducted immediately after subcutaneous injection of fluorescing cells into small animals.

Paper Details

Date Published: 1 July 2008
PDF: 10 pages
J. Biomed. Opt. 13(4) 041310 doi: 10.1117/1.2953528
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Ilya V. Turchin, Institute of Applied Physics (Russian Federation)
Vladislav Antonievich Kamensky, Institute of Applied Physics (Russian Federation)
Vladimir I. Plehanov, Institute of Applied Physics (Russian Federation)
Anna Gennadjevna Orlova, Institute of Applied Physics (Russian Federation)
Mikhail S. Kleshnin, Institute of Applied Physics (Russian Federation)
Ilya I. Fiks, Institute of Applied Physics (Russian Federation)
Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)
Irina G. Meerovich, A.N. Bach Institute of Biochemistry (Russian Federation)
Lyaisan R. Arslanbaeva, A.N. Bach Institute of Biochemistry (Russian Federation)
Viktoria V. Jerdeva, A.N. Bach Institute of Biochemistry (Russian Federation)
Alexander P. Savitsky, A.N. Bach Institute of Biochemistry (Russian Federation)


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