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Journal of Biomedical Optics • Open Access

Raman microscopy for dynamic molecular imaging of living cells

Paper Abstract

We demonstrate dynamic imaging of molecular distribution in unstained living cells using Raman scattering. By combining slit-scanning detection and optimizing the excitation wavelength, we imaged the dynamic molecular distributions of cytochrome c, protein beta sheets, and lipids in unstained HeLa cells with a temporal resolution of 3 minutes. We found that 532-nm excitation can be used to generate strong Raman scattering signals and to suppress autofluorescence that typically obscures Raman signals. With this technique, we reveal time-resolved distributions of cytochrome c and other biomolecules in living cells in the process of cytokinesis without the need for fluorescent labels or markers.

Paper Details

Date Published: 1 July 2008
PDF: 4 pages
J. Biomed. Opt. 13(4) 044027 doi: 10.1117/1.2952192
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Keisaku Hamada, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Nicholas Isaac Smith, Osaka Univ. (Japan)
Minoru Kobayashi, Nanophoton Corp. (Japan)
Yasushi Inouye, Osaka Univ. (Japan)
Satoshi Kawata, Osaka Univ. (Japan)

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