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Journal of Biomedical Optics

Resonance Raman measurements of carotenoids using light-emitting diodes
Author(s): Scott D. Bergeson; Justin B. Peatross; N. Jay Eyring; John F. Fralick; D. N. Stevenson; Scott B. Ferguson
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Paper Abstract

We report on the development of a compact commercial instrument for measuring carotenoids in skin tissue. The instrument uses two light-emitting diodes (LEDs) for dual-wavelength excitation and four photomultiplier tubes for multichannel detection. Bandpass filters are used to select the excitation detection wavelengths. The f/1.3 optical system has high optical throughput and single photon sensitivity, both of which are crucial in LED-based Raman measurements. We employ a signal processing technique that compensates for detector drift and error. The sensitivity and reproducibility of the LED Raman instrument compares favorably to laser-based Raman spectrometers. This compact, portable instrument is used for noninvasive measurement of carotenoid molecules in human skin with a repeatability better than 10%.

Paper Details

Date Published: 1 July 2008
PDF: 6 pages
J. Biomed. Opt. 13(4) 044026 doi: 10.1117/1.2952075
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Scott D. Bergeson, Brigham Young Univ. (United States)
Justin B. Peatross, Brigham Young Univ. (United States)
N. Jay Eyring, Pharmanex (United States)
John F. Fralick, Pharmanex (United States)
D. N. Stevenson, Pharmanex (United States)
Scott B. Ferguson, Pharmanex (United States)

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