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Journal of Biomedical Optics

Multispectral confocal microendoscope for in vivo and in situ imaging
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Paper Abstract

We describe the design and operation of a multispectral confocal microendoscope. This fiber-based fluorescence imaging system consists of a slit-scan confocal microscope coupled to an imaging catheter that is designed to be minimally invasive and allow for cellular level imaging in vivo. The system can operate in two imaging modes. The grayscale mode of operation provides high resolution real-time in vivo images showing the intensity of fluorescent signal from the specimen. The multispectral mode of operation uses a prism as a dispersive element to collect a full multispectral image of the fluorescence emission. The instrument can switch back and forth nearly instantaneously between the two imaging modes (less than half a second). In the current configuration, the multispectral confocal microendoscope achieves 3-μm lateral resolution and 30-μm axial resolution. The system records light from 500 to 750 nm, and the minimum resolvable wavelength difference varies from 2.9 to 8.3 nm over this spectral range. Grayscale and multispectral imaging results from ex-vivo human tissues and small animal tissues are presented.

Paper Details

Date Published: 1 July 2008
PDF: 9 pages
J. Biomed. Opt. 13(4) 044016 doi: 10.1117/1.2950313
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Houssine Makhlouf, College of Optical Sciences/The Univ. of Arizona (United States)
Arthur F. Gmitro, The Univ. of Arizona (United States)
Anthony A. Tanbakuchi, The Univ. of Arizona (United States)
Joshua A. Udovich, The Univ. of Arizona (United States)
Andrew R. Rouse, The Univ. of Arizona (United States)

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