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Journal of Biomedical Optics

Simultaneous multispectral reflectance imaging and laser speckle flowmetry of cerebral blood flow and oxygen metabolism in focal cerebral ischemia
Author(s): Phill B. Jones; Hwa Kyuong Shin; David A. Boas; Bradley T. Hyman; Michael J. Moskowitz; Cenk Ayata; Andrew K. Dunn
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Paper Abstract

Real-time investigation of cerebral blood flow (CBF), and oxy- and deoxyhemoglobin concentration (HbO, HbR) dynamics has been difficult until recently due to limited spatial and temporal resolution of techniques like laser Doppler flowmetry and magnetic resonance imaging (MRI). The combination of laser speckle flowmetry (LSF) and multispectral reflectance imaging (MSRI) yields high-resolution spatiotemporal maps of hemodynamic and metabolic changes in response to functional cortical activation. During acute focal cerebral ischemia, changes in HbO and HbR are much larger than in functional activation, resulting in the failure of the Beer-Lambert approximation to yield accurate results. We describe the use of simultaneous LSF and MSRI, using a nonlinear Monte Carlo fitting technique, to record rapid changes in CBF, HbO, HbR, and cerebral metabolic rate of oxygen (CMRO2) during acute focal cerebral ischemia induced by distal middle cerebral artery occlusion (dMCAO) and reperfusion. This technique captures CBF and CMRO2 changes during hemodynamic and metabolic events with high temporal and spatial resolution through the intact skull and demonstrates the utility of simultaneous LSF and MSRI in mouse models of cerebrovascular disease.

Paper Details

Date Published: 1 July 2008
PDF: 11 pages
J. Biomed. Opt. 13(4) 044007 doi: 10.1117/1.2950312
Published in: Journal of Biomedical Optics Volume 13, Issue 4
Show Author Affiliations
Phill B. Jones, Massachusetts General Hospital (United States)
Hwa Kyuong Shin, Massachusetts General Hospital (United States)
David A. Boas, Massachusetts General Hospital (United States)
Bradley T. Hyman, Massachusetts General Hospital (United States)
Michael J. Moskowitz, Massachusetts General Hospital (United States)
Cenk Ayata, Massachusetts General Hospital (United States)
Andrew K. Dunn, The Univ. of Texas at Austin (United States)

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