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Journal of Biomedical Optics

Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy
Author(s): Hewang Li; Hui-Fang Li; Robin A. Felder; Ammasi Periasamy; Pedro A. Jose
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Paper Abstract

The recycling of G-protein-coupled receptors (GPCR) to the cell surface after internalization plays an important role in the regulation of overall GPCR activity. The angiotensin II type I receptor (AT1R) belongs to class B GPCRs that recycle slowly back to the cell surface. Previous studies have proposed that Rab11 controls the recycling of AT1R; however, recent reports show that Rab4, a rapid recycling regulator, co-localizes also with internalized AT1R. Different from the subcellular co-localization provided by fluorescence microscopy, fluorescence resonance energy transfer (FRET) microscopy provided the spatial relationship of AT1R with Rab4 and Rab11 in the nanometer-range proximity during the entire course of AT1R recycling. During the early recycling stage, internalized AT1Rs were mainly associated with Rab4 in the cytoplasm. During the mid-recycling stage, AT1Rs were associated with both Rab4 and Rab11 in the perinuclear compartments. However, during the late-recycling stage, AT1Rs were mainly associated with Rab11, both in the perinuclear compartments and the plasma membrane. Co-immunoprecipitation data confirmed these dynamic associations, which were disrupted by silencing of either the Rab4 or Rab11 gene. Based on these observations, we propose a Rab4 and Rab11 coordinated model for AT1R recycling.

Paper Details

Date Published: 1 May 2008
PDF: 10 pages
J. Biomed. Opt. 13(3) 031206 doi: 10.1117/1.2943286
Published in: Journal of Biomedical Optics Volume 13, Issue 3
Show Author Affiliations
Hewang Li, Georgetown Univ. Medical Ctr. (United States)
Hui-Fang Li, Georgetown Univ. Medical Ctr. (United States)
Robin A. Felder, Univ. of Virginia (United States)
Ammasi Periasamy, Univ. of Virginia (United States)
Pedro A. Jose, Georgetown Univ. Medical Ctr. (United States)


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