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Journal of Biomedical Optics

Ex vivo multiphoton analysis of rabbit corneal wound healing following conductive keratoplasty
Author(s): Tsung-Jen Wang; Wen Lo; Chiu-Mei Hsueh; Chen-Yuan Dong; Fung-Rong Hu
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Paper Abstract

Ex vivo multiphoton imaging is used to characterize rabbit corneal wound healing after conductive keratoplasty (CK) procedures. CK is performed on the right eyes from eight New Zealand albino rabbits while the left eyes are punctured by a keratoplast tip without energy application. Rabbits are humanely sacrificed 1 day, 1, 2, and 4 weeks after the CK procedure. Eye balls are enucleated and placed on the microscope for multiphoton imaging. Multiphoton imaging reveals damage of corneal epithelium and stroma caused by the CK procedure and the subsequent wound healing process can be followed without histological procedures. Multiphoton excited autofluorescence images demonstrate that re-epithelilialization is accomplished within 1 week in both CK and control groups. However, epithelial hyperplasia is observed in CK corneas. In addition, stromal wounds in the control group become inconspicuous within 1 week while obvious wounds still exist in CK corneas for at least 4 weeks. Postconductive keratoplasty corneal damage and wound healing can be characterized by multiphoton microscopy without histological procedures. Our results suggest that multiphoton microscopy has potential in the clinical evaluation of corneal damage due to refractive surgery, and can be used to study and reduce the unwanted side effects of these procedures.

Paper Details

Date Published: 1 May 2008
PDF: 0 pages
J. Biomed. Opt. 13(3) 034019 doi: 10.1117/1.2943156
Published in: Journal of Biomedical Optics Volume 13, Issue 3
Show Author Affiliations
Tsung-Jen Wang, Taipei Medical Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Chiu-Mei Hsueh, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Fung-Rong Hu, National Taiwan Univ. Hospital (Taiwan)

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