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Journal of Biomedical Optics

Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes
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Paper Abstract

Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.

Paper Details

Date Published: 1 May 2008
PDF: 7 pages
J. Biomed. Opt. 13(3) 031217 doi: 10.1117/1.2942373
Published in: Journal of Biomedical Optics Volume 13, Issue 3
Show Author Affiliations
Xiaochao Qu, Xi'an Jiaotong Univ. (China)
Jing Wang, Xi'an Jiaotong Univ. (China)
Zhenxi Zhang, Xi'an Jiaotong Univ. (China)
Norbert Koop, Univ. zu Lübeck (Germany)
Ramtin Rahmanzadeh, Univ. zu Lübeck (Germany)
Gereon Huettmann, Univ. zu Lübeck (Germany)


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