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Journal of Biomedical Optics

In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation
Author(s): Wataru Watanabe; Sachhiro Matsunaga; Tsunehito Higashi; Kiichi Fukui; Kazuyoshi Itoh
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Paper Abstract

Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.

Paper Details

Date Published: 1 May 2008
PDF: 8 pages
J. Biomed. Opt. 13(3) 031213 doi: 10.1117/1.2939401
Published in: Journal of Biomedical Optics Volume 13, Issue 3
Show Author Affiliations
Wataru Watanabe, National Institute of Advanced Industrial Science and Technology (Japan)
Sachhiro Matsunaga, Osaka Univ. (Japan)
Tsunehito Higashi, Fukushima Medical Univ. (Japan)
Kiichi Fukui, Osaka Univ. (Japan)
Kazuyoshi Itoh, Osaka Univ. (Japan)

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