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Journal of Biomedical Optics

Optical clearing of flowing blood using dextrans with spectral domain optical coherence tomography
Author(s): Xiangqun Xu; Lingfeng Yu; Zhongping Chen
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Paper Abstract

Spectral domain optical coherence tomography (SDOCT) images have been used to investigate the mechanism of optical clearing in flowing blood using dextrans. The depth reflectivity profiles from SDOCT indicate that dextrans become increasingly more effective in reducing scattering in flowing blood, except for 5 mgdl-1 of D×500, with increasing molecular weights (MW 70,000 and 500,000) and concentrations (0.6, 2, and 5 mgdl-1). Among the tested dextrans, Dx500 at 2 mgdl-1 had the most significant effect on light scattering reduction with the strongest capability to induce erythrocyte aggregation. D×500 at 5 mgdl-1 contributes more refractive index matching but induces a decrease in aggregation that leads to the same level as 0.6 mgdl-1 D×500. Previous studies identified various mechanisms of light scattering reduction in stationary blood induced by optical clearing agents. Our results suggest that erythrocyte aggregation is a more important mechanism for optical clearing in flowing blood using dextrans, providing a rational design basis for effective flowing blood optical clearing, which is essential for improving OCT imaging capability through flowing blood.

Paper Details

Date Published: 1 March 2008
PDF: 6 pages
J. Biomed. Opt. 13(2) 021107 doi: 10.1117/1.2909673
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Xiangqun Xu, Zhejiang of Science and Technology Univ. (China)
Lingfeng Yu, Univ. of California/Irvine (United States)
Zhongping Chen, Univ. of California/Irvine (United States)

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