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Journal of Biomedical Optics

Measurements of light scattering in an integrated microfluidic waveguide cytometer
Author(s): Xuan-Tao Su; Kirat Singh; Clarence E. Capjack; Jiri Petracek; Christopher J. Backhouse; Wojciech Rozmus
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Paper Abstract

An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.

Paper Details

Date Published: 1 March 2008
PDF: 10 pages
J. Biomed. Opt. 13(2) 024024 doi: 10.1117/1.2909670
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Xuan-Tao Su, Univ. of Alberta (Canada)
Kirat Singh, Univ. of Alberta (Canada)
Clarence E. Capjack, Univ. of Alberta (Canada)
Jiri Petracek, Brno Univ. of Technology (Czech Republic)
Christopher J. Backhouse, Univ. of Alberta (Canada)
Wojciech Rozmus, Univ. of Alberta (Canada)

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