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Journal of Biomedical Optics

Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography
Author(s): Mark Oldham; Harshad Sakhalkar; Tim Oliver; G. Allan Johnson; Mark W. Dewhirst
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Paper Abstract

Optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are new techniques that enable unprecedented high-resolution 3-D multimodal imaging of tissue structure and function. Applications include imaging macroscopic gene expression and microvasculature structure in unsectioned biological specimens up to 8 cm3. A key requisite for these imaging techniques is effective sample preparation including optical clearing, which enables light transport through the sample while preserving the signal (either light absorbing stain or fluorescent proteins) in representative form. We review recent developments in optical-CT and optical-ECT, and compatible "fluorescence-friendly" optical clearing protocols.

Paper Details

Date Published: 1 March 2008
PDF: 8 pages
J. Biomed. Opt. 13(2) 021113 doi: 10.1117/1.2907968
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Mark Oldham, Duke Univ. Medical Ctr. (United States)
Harshad Sakhalkar, Duke Univ. Medical Ctr. (United States)
Tim Oliver, Duke Univ. Medical Ctr. (United States)
G. Allan Johnson, Duke Univ. Medical Ctr. (United States)
Mark W. Dewhirst, Duke Univ. Medical Ctr. (United States)

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