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Journal of Biomedical Optics • Open Access

Quantitative phase microscopy through differential interference imaging

Paper Abstract

An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.

Paper Details

Date Published: 1 March 2008
PDF: 10 pages
J. Biomed. Opt. 13(2) 024020 doi: 10.1117/1.2907328
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Sharon V. King, Univ. of Colorado at Boulder (United States)
Ariel R. Libertun, Univ. of Colorado at Boulder (United States)
Rafael Piestun, Univ. of Colorado at Boulder (United States)
Carol J. Cogswell, Univ. of Colorado at Boulder (United States)
Chrysanthe Preza, The Univ. of Memphis (United States)

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