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Journal of Biomedical Optics

Short-term and long-term effects of optical clearing agents on blood vessels in chick chorioallantoic membrane
Author(s): Dan Zhu; Jing Zhang; Han Cui; Zongzhen Mao; Pengcheng Li; Qingming Luo
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Paper Abstract

The tissue optical clearing technique shows great potential in optical diagnosis and therapy. However, short-term and long-term effects of optical clearing agents on blood vessels, which are relevant to the safety of clinical applications, have not been clarified. We used laser speckle contrast imaging to monitor the changes in blood vessels in chick chorioallantoic membrane (CAM) after application of glycerol or glucose. The changes in morphology of vessels and blood flow velocity were measured. Long-term effects on blood vessels were investigated by observing the function and the development of blood vessels. The results show that glycerol reduces the local blood flow velocity and constricts and even blocks vessels quickly. At 2 days, the blood flow velocity is recovered to different extents, and new blood vessels develop but are fewer in number. Glucose induces slow changes in blood flow or vessels. However, most blood vessels are blocked, and no new blood vessel develops at 2 days. The effects depend on the dosage of agents, including volume and concentration, and decrease with the dosage of agents. Therefore, short-term effects of glucose on blood vessels are slighter than those of glycerol, but long-term effects of glucose are greater.

Paper Details

Date Published: 1 March 2008
PDF: 8 pages
J. Biomed. Opt. 13(2) 021106 doi: 10.1117/1.2907169
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Dan Zhu, Huazhong Univ. of Science and Technology (China)
Jing Zhang, Huazhong Univ. of Science and Technology (China)
Han Cui, Huazhong Univ. of Science and Technology (China)
Zongzhen Mao, Huazhong Univ. of Science and Technology (China)
Pengcheng Li, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


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