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Journal of Biomedical Optics

Monitoring singlet oxygen in situ with delayed chemiluminescence to deduce the effect of photodynamic therapy
Author(s): Yanchun Wei; Xing Da; Shiming Luo; Wei Xu; Qun Chen
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Paper Abstract

Singlet oxygen (1O2) is an important factor mediating cell killing in photodynamic therapy (PDT). We previously reported that chemiluminescence (CL) can be used to detect 1O2 production in PDT and linked the signal to the PDT-induced cytotoxicity in vitro. We develop a new CL detection apparatus to achieve in vivo measurements. The system utilizes a time-delayed CL signal to overcome the interference from scattered excitation light, thus greatly improving the accuracy of the detection. The system is tested on healthy skin of BALB/ca mouse for its feasibility and reliability. The CL measurement is made during a synchronized gating period of the irradiation light. After each PDT treatment and in situ CL measurement, the skin response is scored over a period of 2 weeks. A remarkable relationship is observed between the score and the CL, regardless of the PDT treatment protocol. Although there are many issues yet to be addressed, our results clearly demonstrate the feasibility of CL measurement during PDT and its potential for in vivo PDT dosimetry. This requires further investigations.

Paper Details

Date Published: 1 March 2008
PDF: 7 pages
J. Biomed. Opt. 13(2) 024023 doi: 10.1117/1.2904961
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Yanchun Wei, South China Normal Univ. (China)
Xing Da, South China Normal Univ. (China)
Shiming Luo, South China Normal Univ. (China)
Wei Xu, South China Normal Univ. (China)
Qun Chen, Univ. of Colorado at Denver (United States)

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