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Journal of Biomedical Optics • Open Access

Fast differential interference contrast imaging combined with autocorrelation treatments to measure the heart rate of embryonic fish
Author(s): Jingtao Zhu; Jia He; Ji Yao Chen; Da-Ru Lu; Luwei Zhou

Paper Abstract

To develop an accurate and convenient method for measuring the heart rate of zebra fish in vivo, a system combining fast differential interference contrast (DIC) imaging with an autocorrelation technique is established. The imaging correlation coefficient corr(i,j) between frame i, selected from the obtained time-lapse imaging series as the reference image, and any other frame j, is calculated as the time-dependent cycle course. Heat rate is determined by the cycle period of the corr with a high temporal resolution of 4 ms, achieved by fast charge-coupled device (CCD) imaging of 250 frames per second. With this high-resolution system, we find that 1-mg/L cadmium not only induces the slowing of the heart rate, but also caused signs of arrhythmia in treated fish.

Paper Details

Date Published: 1 March 2008
PDF: 3 pages
J. Biomed. Opt. 13(2) 020503 doi: 10.1117/1.2904667
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Jingtao Zhu, Tongji Univ. (China)
Jia He, Fudan Univ. (China)
Ji Yao Chen, Fudan Univ. (China)
Da-Ru Lu, Fudan Univ. (China)
Luwei Zhou, Fudan Univ. (China)

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