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Journal of Biomedical Optics • Open Access

Use of scaling relations to extract intrinsic fluorescence lifetime of targets embedded in turbid media

Paper Abstract

We present a novel method for estimating the intrinsic fluorescence lifetime of deeply embedded localized fluorophores. It is based on scaling relations, characteristic for turbid media. The approach is experimentally substantiated by successfully reconstructing lifetimes for targets at depths up to 14.5 mm. A derived correction factor was determined from the product of the transport-corrected scattering coefficient μs and the index of refraction nr. In addition, data from an array of detectors (≥2) can be used to estimate μs nr. The suggested algorithm is a promising tool for diagnostic fluorescence, since lifetime can be a sensitive indicator of the fluorophore environment.

Paper Details

Date Published: 1 March 2008
PDF: 4 pages
J. Biomed. Opt. 13(2) 024025 doi: 10.1117/1.2904660
Published in: Journal of Biomedical Optics Volume 13, Issue 2
Show Author Affiliations
Victor V. Chernomordik, National Institutes of Health (United States)
Moinuddin Hassan, National Institutes of Health (United States)
Franck Amyot, National Institutes of Health (United States)
Jason D. Riley, National Institutes of Health (United States)
Amir H. Gandjbakhche, National Institutes of Health (United States)


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